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Installation

This document is WIP and still a mixture of old and new docs.

Large system deployments can get very complex. In this document we explain the GeneNetwork reproducible deployment system which is based on GNU Guix The Guix system can be used to install GN with all its files and dependencies.

Note that the official deployment works through a Guix VM. This is described in

Check list

To run GeneNetwork the following services need to function:

Installing Guix packages

Make sure to install GNU Guix using the binary download instructions on the main website. Follow the instructions on Note the download amounts to several GBs of data. Debian-derived distros may support

apt-get install guix

Creating a GNU Guix profile

We run a GNU Guix channel with packages at

The README has instructions hosting a channel (recommended!), but sometimes we use the GUIX_PACKAGE_PATH instead. First upgrade to a recent guix with

mkdir ~/opt
guix pull -p ~/opt/guix-pull

It should upgrade (ignore the locales warnings). You can optionally specify the specific git checkout of guix with

guix pull -p ~/opt/guix-pull --commit=f04883d

which is useful when you need to roll back to an earlier version (sometimes our channel goes out of sync). Next, we install GeneNetwork2 with

source ~/opt/guix-pull/etc/profile
git clone https://git.genenetwork.org/guix-bioinformatics/guix-bioinformatics.git ~/guix-bioinformatics

you probably also need guix-past (the upstream channel for older packages):

git clone https://gitlab.inria.fr/guix-hpc/guix-past.git ~/guix-past
cd ~/guix-past
env GUIX_PACKAGE_PATH=$HOME/guix-bioinformatics:$HOME/guix-past/modules ~/opt/guix-pull/bin/guix package -i genenetwork2 -p ~/opt/genenetwork2

Ignore the warnings. Guix should install the software without trying to build everything. If you system insists on building all packages, try the `--dry-run` switch and fix the [[https://guix.gnu.org/manual/en/html_node/Substitute-Server-Authorization.html][substitutes]]. You may add the `--substitute-urls="http://guix.genenetwork.org https://ci.guix.gnu.org https://mirror.hydra.gnu.org"` switch.

The guix.genenetwork.org has most of our packages pre-built(!). To use it on your own machine the public key is

(public-key
 (ecc
  (curve Ed25519)
  (q #9F56EAB5CE37AA15693C31F451140588240F259676C137E31C0CA70EC4D1B534#)
  )
 )

Once we have a GNU Guix profile, a running database (see below) and the file storage, we should be ready to fire up GeneNetwork:

Running GN2

Check out the source with git:

git clone git@github.com:genenetwork/genenetwork2.git
cd genenetwork2

You may want to use the testing branch.

Run GN2 with earlier created Guix profile

export GN2_PROFILE=$HOME/opt/genenetwork2
env TMPDIR=$HOME/tmp WEBSERVER_MODE=DEBUG LOG_LEVEL=DEBUG SERVER_PORT=5012 GENENETWORK_FILES=/export/data/genenetwork/genotype_files SQL_URI=mysql://webqtlout:webqtlout@localhost/db_webqtl ./bin/genenetwork2 etc/default_settings.py -gunicorn-dev

The script comes with debug and logging switches can be particularly useful when developing GN2. Location and files are examples.

It may be useful to tunnel the web server to your local browser with an ssh tunnel:

Testing on an ssh tunnnel

If you want to test a service running on the server on a certain port (say 8202) use

ssh -L 8202:127.0.0.1:8202 -f -N myname@penguin2.genenetwork.org

And browse on your local machine to http://localhost:8202/

BELOW INFORMATION NEEDS TO BE UPDATED

GeneNetwork requires a separate gn-proxy server which handles authorisation and access control. For instructions see the [[https://github.com/genenetwork/gn-proxy][README]]. Note it may already be running on our servers!

Redis part of GN2 deployment and will be started by the ./bin/genenetwork2 startup script.

** Install MariaDB with GNU GUIx

These are the steps you can take to install a fresh installation of mariadb (which comes as part of the GNU Guix genenetwork2 install).

As root configure the Guix profile, previously that was

. ~/opt/genenetwork2/etc/profile

But now we use the recommended

/usr/local/guix-profiles/guix-pull/bin/guix install mariadb borg -p /usr/local/guix-profiles/gn-latest
. /usr/local/guix-profiles/gn-latest/etc/profile

Exctract the db (that takes a while too)

/usr/local/guix-profiles/gn-latest/bin/borg extract /export2/data/wrk/tux01/borg-tux01::borg-backup-mariadb-20240218-06:16-Sun --progress

and run for example

adduser mariadb
addgroup mariadb (and add user to group)
mkdir -p /export2/mariadb/database
chown mariadb.mariadb -R /export2/mariadb/
mkdir -p /var/run/mysqld
chown mariadb.mariadb /var/run/mysqld
su mariadb
. /usr/local/guix-profiles/gn-latest/etc/profile
mariadb --version
  mariadb  Ver 15.1 Distrib 10.10.2-MariaDB, for Linux (x86_64) using readline 5.1
mariadb_install_db --user=mariadb --datadir=/export2/mariadb/database
mariadbd -u mariadb --datadir=/exportdb/mariadb/database/mariadb --explicit_defaults_for_timestamp -P 12048"

If you want to run as root you may have to set /etc/my.cnf

[mariadbd]
user=root

You also need to set

ft_min_word_len = 3

To make sure word text searches (shh) work and rebuild the tables if required.

To check error output in a file on start-up run with something like

mariadbd -u mariadb --console  --explicit_defaults_for_timestamp  --datadir=/export/mariadb/tux01_mariadb/latest --log-error=~/test.log
/usr/local/guix-profiles/gn-latest/bin/mariadb -uwebqtlout -pwebqtlout db_webqtl -e 'show tables'

When you get errors like:

qlalchemy.exc.IntegrityError: (_mariadb_exceptions.IntegrityError) (1215, 'Cannot add foreign key constraint')

you may need to set

set foreign_key_checks=0

The current production my.conf is

[mysqld]

# innodb_empty_free_list_algorithm=backoff
innodb_buffer_pool_size=16G
# innodb_ibuf_max_size=2G
innodb_ft_min_token_size=3
# innodb_use_sys_malloc=0
innodb_file_per_table=ON
key_buffer_size=10M

ft_min_word_len = 3

# main = 1 for active master: server A
gtid-domain-id=1

tmpdir=/export/tmp
wait_timeout=180
lc_time_names=en_US
lc_messages=en_US
max_connections=2048
thread_cache_size=16
open_files_limit = 16384
query_cache_type=1                                                                                  query_cache_min_res_unit = 1k
query_cache_limit = 1M
query_cache_size=128M

log_error=/var/log/mysql/error.log
skip-name-resolve

# Skip recovery for now:
innodb_force_recovery=1

# Only when listening to the network!
# bind-address = 0.0.0.0
# port = 3306

slow_query_log=1
slow_query_log_file=/var/log/mysql/mysql-slow.log
long_query_time=60.0
log_queries_not_using_indexes=0
log_warnings=4
log_slow_admin_statements=ON

ft_min_word_len=3

log-bin=/var/lib/mysql/gn0-binary-log
expire-logs-days=120
server-id=1
# Domain = 1 for active master: server A
gtid-domain-id=1

[myisamchk]
sort_buffer_size=4M
ft_min_word_len = 3

Note that we handle IP restrictions through the nftables firewall.

The systemd config is

[Unit]
Description=MariaDB database server
Documentation=man:mysqld(8)
Documentation=https://mariadb.com/kb/en/library/systemd/
After=network.target

[Install]
WantedBy=multi-user.target
Alias=mysqld.service

[Service]
TimeoutStartSec=infinity
TimeoutStopSec=infinity
LimitNOFILE=infinity
LimitMEMLOCK=infinity

Type=simple
PrivateNetwork=false

User=mariadb
Group=mariadb

CapabilityBoundingSet=CAP_IPC_LOCK                                                                  # Prevent writes to /usr, /boot, and /etc
ProtectSystem=true
                                                                       PrivateDevices=true
# Prevent accessing /home, /root and /run/user
ProtectHome=false

# Execute pre and post scripts as root, otherwise it does it as User=
PermissionsStartOnly=true

ExecStartPre=/usr/bin/install -m 755 -o mariadb -g root -d /var/run/mysqld

ExecStart=/usr/local/guix-profiles/gn-latest/bin/mariadbd --datadir=/export/mariadb/tux01_mariadb/latest $MYSQLD_OPTS $_WSREP_NEW_CLUSTER $_WS
REP_START_POSITION -W

ExecStartPost=/bin/sh -c "systemctl unset-environment _WSREP_START_POSITION"

KillSignal=SIGTERM

SendSIGKILL=no
Restart=on-abort
RestartSec=15s

UMask=007

PrivateTmp=false

** Load the small database in MySQL

Currently we have two databases for deployment, 'db_webqtl_s' is the small testing database containing experiments from BXD mice and 'db_webqtl_plant' which contains all plant related material.

Download one database from

After installation unzip the database binary in the MySQL directory, e.g.

cd ~/mysql
p7zip -d db_webqtl_s.7z
chown -R mysql:mysql db_webqtl_s/
chmod 700 db_webqtl_s/
chmod 660 db_webqtl_s/*

restart MySQL service (mysqld). Login as root

: mysql_upgrade -u root --force

: myslq -u root

and

: mysql> show databases; : +--------------------+ : | Database | : +--------------------+ : | information_schema | : | db_webqtl_s | : | mysql | : | performance_schema | : +--------------------+

Set permissions and match password in your settings file below:

: mysql> grant all privileges on db_webqtl_s.* to gn2@"localhost" identified by 'webqtl';

You may need to change "localhost" to whatever domain you are connecting from (mysql will give an error).

Note that if the mysql connection is not working, try connecting to the IP address and check server firewall, hosts.allow and mysql IP configuration (see below).

Note for the plant database you can rename it to db_webqtl_s, or change the settings in etc/default_settings.py to match your path.

Get genotype files

The script looks for genotype files. You can find a BXD subset in

(Note we should add plants)

mkdir -p $HOME/genotype_files
cd $HOME/genotype_files

Trouble shooting

Mysql can't connect server through socket ERROR

The following error

: sqlalchemy.exc.OperationalError: (_mysql_exceptions.OperationalError) (2002, 'Can\'t connect to local MySQL server through socket \'/run/mysqld/mysqld.sock\' (2 "No such file or directory")')

means that MySQL is trying to connect locally to a non-existent MySQL server, something you may see in a container. Typically replicated with something like

: mysql -h localhost

try to connect over the network interface instead, e.g.

: mysql -h 127.0.0.1

if that works run genenetwork after setting SQL_URI to something like

: export SQL_URI=mysql://gn2:mysql_password@127.0.0.1/db_webqtl_s

(made with skribilo)